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Earlier sources: Biotechnology
This science thread follows biotechnology through original research, public projects, and source reports. Read the evidence below to see which developments are represented in SYMTRI’s archive.
SYMTRI organizes observations from public sources into a living map. Connections reflect shared subject matter; they do not establish causation or endorse a source.
Open in the map ↗229 classified signals in the fourteen-day window. These counts include signals awaiting readable evidence.
Earlier sources
Up to ten sources with readable evidence, ordered by their feed timestamp. These archive sources can be older than the live fourteen-day window. This list is a sample, not a measure of total activity. Dates distinguish paper publication, repository creation, model/dataset activity, Hacker News posts, and KEV catalog additions. “First archived” records when SYMTRI first stored the signal.
- OpenAlex · Published · First archived
RuPHOTACs provide photocontrol over protein degradation with optimized properties for biological applications ↗
PROteolysis TArgeting Chimeras (PROTACs) are bifunctional molecules that catalyze degradation of selected proteins by inducing protein:protein interactions (PPIs) between E3 ubiquitin ligases and the protein of interest. A critical limitation is undesired effects in untargeted tissues, necessitating approaches to impose spatiotemporal control over PROTAC function. Here we present Ru(II) photocages that can be released with low energy light, providing triggered PROTAC activity on demand. The systems, termed Ruthenium-based PHOToActivated Chimeras (RuPHOTACs), were validated by targeting bromodo…
- OpenAlex · Published · First archived
Genome-Wide Identification of the PP2A Gene Family in Strawberry (Fragaria × ananassa) and Pharmacological Evidence for the Involvement of PP2A in ALA-Mediated Salt Tolerance ↗
Background: Protein phosphatase 2A (PP2A) is a conserved serine/threonine phosphatase that plays crucial roles in stress responses, yet its function in strawberry (Fragaria spp.) remains largely uncharacterized. This study aimed to characterize the PP2A gene family in octoploid strawberry and to investigate its involvement in ALA-induced salt tolerance. Methods: We identified PP2A genes in the octoploid strawberry ‘Benihoppe’ genome and classified them into scaffold (A), regulatory (B55, B56, B″), and catalytic (C) subunit families. Segmental duplication and selection pressure were analyzed us…
- OpenAlex · Published · First archived
Programmable DNA protonuclei reveal environmental context on protein phase separation ↗
Abstract Understanding protein phase separation in cellular environments remains a major challenge, as ex vivo assays often fail to capture the influence of environmental context – such as crowding, multimodal interactions, and the dynamic properties of the cytosol or nucleus. Here, we introduce programmable DNA-based protonuclei (PN) as nucleus-inspired compartments to probe phase separation of the neurodegeneration-linked protein FUS. We show that FUS partitioning and condensate formation are highly sensitive to nucleic acid sequence, spatial confinement, and viscoelastic properties of the P…
- OpenAlex · Published · First archived
Silver(I)-mediated amide-to-ester transformation on unprotected peptides for protein chemical synthesis and engineering ↗
Chemical protein synthesis has transformed the preparation of homogeneous peptides and proteins with site-specific modifications. Serine/threonine ligation offers a practical route to access difficult peptides and proteins via its aggregation-breaking intermediate, yet the preparation and regeneration of the prerequisite peptidyl salicylaldehyde ester still require the development of more efficient chemistry. Here, we report a silver(I)-mediated amide-to-ester transformation on unprotected peptides, enabled by an easily introduced N , S -benzylidene thioacetal scaffold. This method can chemose…
- OpenAlex · Published · First archived
Genome-Wide Discovery and Evaluation of Kazakh Ancestry-Informative Markers ↗
Kazakh populations occupy an important position within the genetic landscape of Central Asia and Inner Eurasia, yet compact ancestry-informative marker panels evaluated for Kazakh-related ancestry remain limited. We analyzed genome-wide single-nucleotide polymorphism data from 624 Kazakh individuals together with data from external reference populations. After harmonization, the discovery dataset comprised 1607 individuals from 41 population groups and 80,766 shared autosomal variants. Candidate ancestry-informative markers were ranked using allele-frequency differentiation and informativeness…
- OpenAlex · Published · First archived
Galactokinase 1 Positively Regulates Mitochondrial Respiration by Phosphorylating TIMM13 as a Protein Kinase ↗
ABSTRACT Galactokinase 1 (GALK1) is a metabolic kinase that phosphorylates galactose. Mutations in GALK1 cause type II galactosemia, which manifests as cataracts and extra‐ocular symptoms. The mechanisms underlying these symptoms are not fully understood. In this study, we explored a novel function of GALK1 beyond galactose phosphorylation. GALK1 is ubiquitously expressed across human tissues, regardless of developmental stage. GALK1 phosphorylates TIMM13 at the Y73 residue. The phosphorylated Y73 stabilizes TIMM13 by electrostatically interacting with two cationic residues, thereby enhancing…
- OpenAlex · Published · First archived
Effect of Improved pET Plasmids on Protein Expression Yield and Basal Expression ↗
High-yield recombinant protein expression is a critical requirement in biopharmaceutical development. The pET vector family is among the most widely used systems for protein production in Escherichia coli. Shilling et al. (2020) reported that insertion of a GAGA sequence within the T7lac promoter region of pET28a enhanced protein expression and suggested broad applicability across the pET plasmid family [1]. In this study, the GAGA sequence was introduced into pET16b and pET11a plasmids, and the expression performance of the original and modified vectors was evaluated using two recombinant pro…
- OpenAlex · Published · First archived
Enhancing protein–ligand pose prediction via dual-branch deep learning with docking-oriented multi-component loss and iterative refinement ↗
Abstract Protein-ligand pose prediction is a core task in structure-based drug discovery because it determines how a ligand fits within a protein pocket and directly affects downstream virtual screening and lead-optimization workflows. Recent graph neural network (GNN) methods have shown promise for protein-ligand pose prediction, while improving the accuracy of an initial docked pose remains an important task. In this work, we present KTransPose, a GNN-based protein-ligand pose-refinement framework that combines a dual-branch architecture, a docking-oriented multi-component loss, and iterativ…
- OpenAlex · Published · First archived
Egg white protein alleviated high-fat-diet-induced obesity in mice by modulating lipid metabolism and gut microbiota: novel insights into synergistic effect ↗
Abstract Egg white protein (EWP) is considered an optimal dietary protein for weight management due to its superior amino acid profile, high biological value, and diverse metabolic regulatory functions. The study explored the effects of EWP on lipid metabolism in high-fat diet (HFD)-induced obese mice through transcriptomic and gut microbiota analyses. These results showed that EWP intake significantly influenced body weight, adipose tissue, and glucose tolerance in obese mice. EWP-1200 reduced blood lipid characteristics, decreased adipocyte size and improved hepatic injury. Moreover, EWP sup…
- OpenAlex · Published · First archived
Development of G Protein-Coupled Receptor 183 Benzimidazole Antagonists Utilizing a Core-Hopping Approach ↗
G protein-coupled receptor 183 (GPR183, EBI2) has emerged as a promising therapeutic target for neuropathic pain due to its role in neuroinflammatory signaling. Previously, our group identified SAE-14 as a potent GPR183 antagonist and lead scaffold for analgesic development. To further explore the structural requirements for receptor antagonism, a ligand-based core-hopping strategy was employed to identify novel chemotypes derived from SAE-14. Virtual screening identified a trisubstituted benzimidazole scaffold as a promising candidate for further investigation. A focused series of benzimidazo…
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How to assess evidence about Biotechnology
Check each original source for its methods, release status, limitations, and supporting data. A repository announcement, a preprint, and a published study provide different kinds of evidence. SYMTRI’s subject classification helps discovery; it does not independently validate the source’s claims.
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